环介导等温扩增检测特异性与准确性的改善方法

Methods to improve the detection specificity and accuracy of loop-mediated isothermal amplification (LAMP)

摘要:

环介导等温扩增(loop-mediated isothermal amplification, LAMP)是一种快速、灵敏的核酸等温扩增方法，具有良好的特异性和敏感性，在核酸检测领域具有广阔的应用前景。然而，基于LAMP的检测方案开发也面临一些问题，例如在扩增过程中会出现非特异性和非模板扩增等情况，进而影响检测结果的准确性和特异性。本文阐述了近年来LAMP开发过程中在规避假阳性、提升检测效果等方面的研究进展，包括引物设计、优化条件、引入特殊化学物质等策略，并探讨了LAMP在检测领域的应用突破与创新。

Abstract:

Loop-mediated isothermal amplification (LAMP), a rapid and sensitive method of nucleic acid isothermal amplification, is characterized by high specificity and sensitivity and holds broad application prospects in nucleic acid detection. However, the development of detection schemes based on LAMP encounters issues, such as non-specific and non-template amplification during the amplification process, which can affect the accuracy and specificity of the detection results. This article elaborates on the recent research progress in the strategies avoiding false positives and enhancing detection efficacy of LAMP, which include primer design, optimization of conditions, and introduction of special chemical substances. Finally, this article explores the breakthroughs and innovations of LAMP in the field of detection.