天然产物生物合成中的SnoaL家族蛋白研究进展

Research progress on SnoaL family proteins in natural product biosynthesis

摘要:

SnoaL家族蛋白属于核转运因子2 (nuclear transport factor 2, NTF2)样超家族，具有特征性的α+β折叠桶状结构。近年来研究发现该家族蛋白具有丰富的催化功能，能够催化羟醛缩合、羟化、脱羧、水解以及环加成等多种反应，在多种复杂结构活性天然产物的生物合成中发挥着重要作用。尽管这一家族蛋白广泛分布于细菌、真菌等生物体中，但目前针对该家族蛋白的研究还相对较少。本文通过系统综述SnoaL家族蛋白的研究进展，不仅能为后续相关蛋白的功能鉴定和机制研究提供参考，同时也有助于发现新颖的生物催化工具，为生物活性分子的合成生物学研究奠定基础。

Abstract:

SnoaL family proteins belong to a class of the nuclear transport factor 2 (NTF2)-like superfamily, which are characterized by a distinctive α+β barrel fold structure. To date, proteins of this family have been demonstrated to catalyze a variety of reactions, such as aldol reaction, hydroxylation, decarboxylation, epoxide hydrolysis, and cycloaddition reaction, playing crucial roles in the biosynthesis of complex bioactive natural products. These proteins are widely distributed in various organisms including bacteria and fungi. However, research on proteins of this family is relatively limited at present. We reviewed the research progress on proteins of SnoaL family. The review not only provides beneficial guidance for the subsequent functional identification and mechanism research of related proteins but also helps to discover novel biocatalytic tools, laying a foundation for the research of bioactive molecule biosynthesis.