大肠杆菌抗氟乙酸变株的选育及应用

摘要:

In the cultivation of gene engineered strain of Escherichia coli on glucose medium, excretion and accumulation of acetic acid inhibit not only cell growth but also the the expression of heterologous protein. It is obvious that the desirable host strain maintaining acetate at a low level is one of the approaches to increase the production of recombinant protein. The present article deals with the selection of mutants of E.coli DP19, DP8, which grow on the medium containing pyruvate as the sole carbon…

关键词:氟乙酸; 乙酸; 磷酸转乙酰酶（PTA）; 乙酸激酶（ACK）; GL7ACA酰化酶

Abstract:

In the cultivation of gene engineered strain of Escherichia coli on glucose medium, excretion and accumulation of acetic acid inhibit not only cell growth but also the the expression of heterologous protein. It is obvious that the desirable host strain maintaining acetate at a low level is one of the approaches to increase the production of recombinant protein. The present article deals with the selection of mutants of E.coli DP19, DP8, which grow on the medium containing pyruvate as the sole carbon…

Key words:氟乙酸; 乙酸; 磷酸转乙酰酶（PTA）; 乙酸激酶（ACK）; GL7ACA酰化酶