用TnV分离粘细菌发育回复突变株

摘要:

fruA∷TcΩ5 is a development deficient strain of M.Xamthus.Transposon TnV was used to randomly mutagenize various sites of fruA ∷TcΩ5 chromosome.Fruiting body formation was restored in one TnV insertion mutant,designated XM1206.The TnV\|inserted DNA fragment from XM1206 chromosome was cloned,which may be served as a probe to isolate the corresponding allele from wild\|type strain.

Abstract:

fruA∷TcΩ5 is a development deficient strain of M.Xamthus.Transposon TnV was used to randomly mutagenize various sites of fruA ∷TcΩ5 chromosome.Fruiting body formation was restored in one TnV insertion mutant,designated XM1206.The TnV\|inserted DNA fragment from XM1206 chromosome was cloned,which may be served as a probe to isolate the corresponding allele from wild\|type strain.