ATP生物发光法检测抗菌药敏感性影响因素的研究进展

Influencing factors of ATP bioluminescence-based antimicrobial susceptibility testing: a review

摘要:

抗菌药敏感性试验在抗菌药残留检测、细菌性疾病的预防和治疗领域具有重要意义。传统的抗菌药敏感性试验需要经过细菌培养和菌落计数等过程，耗时且劳力。ATP生物发光法已被广泛用于评估食品和医疗卫生中的细菌污染，似乎是一种快速、灵敏的抗菌药敏感性测试替代技术。本文就ATP生物发光法的性能影响因素进行综述，主要综述这些因素如何影响该方法的检测能力，为临床、环境和食品分析中的抗菌药敏感性分析提供参考。

Abstract:

Antimicrobial susceptibility testing (AST) is of great significance for the detection of antibiotic residues, as well as the prevention and treatment of bacterial diseases. Conventional methods of AST are time-consuming and labor-intensive due to the processes of bacterial culture and colony counting. ATP bioluminescence has been widely employed to assess bacterial contamination in food and healthcare and appears to be a rapid and sensitive alternative to conventional methods of AST. This review illustrates the influencing factors of ATP bioluminescence-based AST in detail. It mainly focuses on how these factors influence the detection performance of the method, giving insights into AST in clinical, environmental, and food analyses.