脱氮除硫菌株的分离鉴定和功能确认

Isolation,identification and characterization of nitrate-reducing and sulfide-oxidizing bacteria

摘要:

从长期稳定运行的脱氮除硫反应器污泥中,分离获得两株具有脱氮除硫功能的芽孢杆菌。经形态观察、生理试验和16SrDNA序列比对,将两菌株归入芽孢杆菌属,菌株CB归类于Bacillus pseudofirmus,菌株CS则与Bacillus hemicellulosilytus和Bacillus halodurans最为接近。以Biolog板检测,菌株CB的基质多样性不明显,菌株CS则可利用Biolog板中多种碳源。菌株CB和菌株CS都能以硝酸盐氧化硫化物,其中菌株CB对硝酸盐、硫化物的转化能力大于CS,菌株CB对硝酸盐的亲和力也大于菌株CS。

Abstract:

Bacterial strains CB and CS were isolated from the Anoxic Sulfide Oxidizing (ASO) reactor working under steady-state with sulfide and nitrate as electron donor and acceptor，respectively. Based on electron microscopy，physiological test and 16S rDNA phylogenetic sequence analysis，the isolate CB was very close to Bacillus pseudofirmus and the isolate CS was very close to Bacillus hemicellulosilytus and Bacillus halodurans. The 16S rDNA sequences of both isolates were submitted to GenBank，NCBI and accession numbers EF542806 and EF542807 were allotted for isolates CB and CS，respectively. According to Biolog carbon source utilization test，the isolate CB is weak to use the carbon sources，while the isolate CB can use many carbon sources. Both the isolate CB and the isolate CS are able to use nitrate for sulfide oxidation. The isolate CS has greater capability to oxidize sulfide with nitrate as electron acceptor.