细菌转录因子调控功能的研究策略

Research strategies for the regulatory functions of bacterial transcription factors

摘要:

转录因子对细菌的生存至关重要，其功能解析有助于推动抗菌药物研发、工业应用以及环境保护等多个领域的发展。目前，转录因子的研究方法与策略种类繁多，在生物信息学和分子生物学等领域均取得了迅速发展。本文从未知转录因子的鉴定方法、转录因子调控功能的鉴定方法、转录因子调控网络的构建方法以及转录因子调控基因的验证方法等4个方面进行综述，为细菌转录因子调控功能的分析与验证提供了新策略与新思路。

Abstract:

Transcription factors are essential for the survival of bacteria, and revealing their functions is conducive to the development of antimicrobials, industrial applications, and environmental protection. At present, there are many methods and strategies for the research on transcription factors, which have been developed rapidly in the fields of bioinformatics and molecular biology. This paper summarizes the methods for identifying unknown transcription factors, unveiling the regulatory functions of transcription factors, constructing regulatory networks of transcription factors, and validating the regulatory genes of transcription factors. This review aims to provide new strategies and new ideas for the analysis and verification of the regulatory functions of bacterial transcription factors.