智舌快速鉴定水产品多种致病性弧菌

Detection of pathogenic Vibrio by Smartongue

摘要:

摘要：【目的】探索研究能很好显示被检测液态食品样品综合信息的电子舌是否也能很好的显示致病性弧菌液体培养物的综合信息，籍此探讨一类致病性弧菌的快速鉴定鉴别新技术。【方法】基于多频脉冲伏安法的智舌，结合主成分分析，对源自水产品的11种致病性弧菌进行区分鉴定研究，得到最佳电极阵列和频率段组合。【结果】结果显示，区分致病性弧菌效果好的电极和频率段分别是：钛电极的100 Hz、银电极的100 Hz和钨电极的1 Hz、10 Hz。钨电极的1 Hz频率段能够独立的把11种致病性弧菌在同一张主成分得分图上区分开；钛电极的100 Hz、银电极的100 Hz和钨电极的10 Hz两两组合也能把11种致病性弧菌主成分得分图上区分开。【结论】研究结果表明，智舌伏安法结合主成分分析法区分致病性弧菌是可行的，具有很大的持续研究价值，有望发展成一类很有发展优势的快速鉴定致病性弧菌乃至延伸至其他致病菌的新技术。

Abstract:

Abstract: [Objective] Electronic tongue is a modern analysis instrument and can detect very well the comprehensive information of liquid samples. Based on this, we employed the electronic tongue to detect the bacteria liquid culture, with the purpose of developing a new rapid method for the detection and identification of food-borne pathogens.[Methods] A novel voltammetric electronic tongue, smart tongue, was used to detect and differentiate 11 species of pathogenic Vibrio. Principal component analysis (PCA) was used to extract the relevant information obtained by the smart tongue. From the data evaluation, the score plots of PCA were obtained. [Results] According to the plots, we chose the most feasible working electrodes at the most favorable frequency segments were determined for our purpose. The results showed that the best electrode arrays and frequency segments to differentiate pathogenic Vibrio were titanium electrode in 100 Hz, silver electrode in 100 Hz and tungsten electrode in 1 Hz and 10 Hz, respectively. We differentiated 11 species of pathogenic Vibrio independently in the score plot of tungsten electrode in 1 Hz. We also distinguished all the Vibrio with the combination of two of other three electrodes and frequencies. [Conclusion] Smartongue could differentiate well 11 species of pathogenic Vibrio from the results analysed by PCA. It has a promising future as a novel modern rapid analytical technology for detecting and distinguishing the pathogenic Vibrio. The method and results could be a good stencil-plate to the research of detecting the other food-borne pathogens with smartongue, and good reference information for the further study.