ε-聚赖氨酸产生菌TUST-2的分离鉴定

Isolation and characterization of a new ε-poly-L-lysine-producing strain TUST-2

摘要:

摘要：【目的】ε-聚赖氨酸是一种天然氨基酸同聚物，本研究目的为分离筛选新的ε-聚赖氨酸产生菌。【方法】采用一种新的分离方法从土壤中分离ε-PL产生菌。分离方法含3步：(1)富集培养ε-PL耐受菌；(2)通过改进的Nishikawa方法筛选；(3)挑选高浓度ε-PL耐受菌株。【结果】从海南省土样中分离获得ε-聚赖氨酸产生菌TUST-2。分类和形态特征属链霉菌属。16S rDNA序列分析比对结果表明TUST-2属淀粉酶产色链霉菌（Streptomyces diastatochromogenes）。经特征反应分析、水解物分析、红外光谱、1H NMR、13C NMR和MALDI-TOF-MS分析表明TUST-2发酵产物为ε-聚赖氨酸。【结论】根据16S rRNA基因序列比对和形态及生理生化特征表明ε-聚赖氨酸产生菌TUST-2属于淀粉酶产色链霉菌，命名为淀粉酶产色链霉菌TUST-2。

Abstract:

Abstract: [Objective] ε-Poly-L-Lysine (ε-PL) is a natural amino acid homopolymer. The study aimed at isolating new ε-PL-producing strains. [Methods] ε-PL-producing strains were screened from the soil by using a new isolation approach which had three steps, (1) enrichment culturing ε-PL toleranting strains; (2) screening by improved Nishikawa’s method; (3) selection of strains with higher ε-PL tolerant ability. [Results]A new ε-PL-producing strain TUST-2 was isolated from the soil collected from Hainan province, China. Chemotaxonomic and morphological characteristics of the isolate were typical of strain of the genus Streptomyces. The strain TUST-2 was found to belong to Streptomyces diastatochromogenes by comparative 16S rRNA gene sequence analysis. The purified fermentation product of the strain TUST-2 was confirmed as ε-PL by characteristic analysis, hydrolysate analysis, infrared spectrum, 1H NMR Spectrum, 13C NMR Spectrum, and MALDI-TOF-MS. [Conclusion] On the basis of 16S rRNA gene sequence analysis and its morphological and physiological characteristics, ε-PL-producing strain TUST-2 is a new isolate of Streptomyces diastatochromogenes, named as Streptomyces diastatochromogenes TUST-2.