醉马草内生菌的分离、鉴定及杀虫研究

Study on the isolation , identification and insecticidal activity of endophyte from Achnatherum inebrians

摘要:

摘要：【目的】探明醉马草内生菌的种类，筛选对农作物虫害有毒杀作用的菌株。【方法】采用研磨法从健康醉马草植物的根、茎、叶和种子中进行菌种分离；通过对其形态、培养特征、生理生化及其他生物学特性的研究；16S rDNA及ITS序列的系统发育学分析进行鉴定；用玻片浸渍法和喷雾法筛选产杀虫活性物质菌株。【结果】获得细菌89株，分别属于枯草芽孢杆菌属（Bacillus）、链霉菌属(Streptomyces)、棒状杆菌属（Corynebacterium）、叶杆菌属（Phyllobacterium）、鞘脂单胞菌属（Sph

Abstract:

Abstract：[Objective] To study the endophyte species of Achnatherum inebrians and to screen strains which have insecticidal activity to cotton insect.[Methods] We isolated endophytic from roots, stems, leaves and seeds of health A. inebrians by grinding separation method and identified by a dual approach of morphological and physiological observation and 16S rDNA gene (for bacteria) and ITS sequence (for fungi) based molecular identification. Then, those endophytes were inoculated into liquid media for fermentation and the crude extracts were used to insecticidal activities tests by slide disc immersion and nebulization methods.[Results] A total of 89 bacteria species that classified into 8 genera of Bacillus, Streptomyces, Corynebacterium, Phyllobacterium, sphingomonnas, Paenibacillus, Pseudomonas, Acinetobacter and 2 fungi of Claviceps purpure and Claviceps Chaetomium, were isolated. Of them, the strain Streptomyces rochei (GA) and Claviceps purpurea (PF-2) were showed more than 85% of mortality to cotton aphis, after primary and second screenings. [Conclusion] Two strains of PF-2 and GA associated within the A. inebrians had significant insecticidal activity to cotton aphis(Aphis gossypii), which may provide a new biological resource to explore new microbial insecticide.