溃烂症眼斑拟石首鱼分离的灿烂弧菌的鉴定

Identification of a Vibrio splendidus strain isolated from diseased Sciaenops ocellatus

摘要:

摘要：【目的】从患溃烂症的眼斑拟石首鱼分离到优势菌株M-1，并进一步对该菌的系统发育学进行了分析。【方法】采用形态学、生理生化鉴定，结合16S rRNA和HSP60基因序列分析。【结果】16S rRNA基因同源性分析，菌株M-1与灿烂弧菌(AJ874361、AY046955)聚为一群；HSP60基因(groES)序列分析表明M-1与弧菌(EU653883、AY837440)聚为一个分支。【结论】菌株M-1属于灿烂弧菌(Vibrio splendidus)。

Abstract:

Abstract: [Objective] Bacterial strain named M-1 was isolated from the diseased Sciaenops ocellatus with ulceration, to further analyze the phylogenetics of the bacterial strain. [Methods] We adopted the characteristics of morphology and physiology, accompanied with the sequence comparison of 16S rRNA and HSP60 genes. [Results] Strain M-1 was identified as Vibrio splendidus. 16SrRNA gene of strain M-1 and Vibrio splendidus(AJ874361) had nearest kinship, and the analysis of HSP60 gene sequence indicated that strain M-1 togethered with Vibrio (EU653883、AY837440)in phylogenetic tree. [Conclusion] The results suggested that strain M-1 was a member of Vibrio splendidus.