细菌ClpX蛋白酶的结构和功能

Bacterial ClpX protease structure and function－A review

摘要:

摘要：ClpX是热休克蛋白Hsp100蛋白家族的成员之一，在生物体中非常保守。Hsp100/ Clp 分子伴侣家族功能主要涉及到细胞对环境的压力耐受、胞内蛋白质的周转、DNA复制和基因表达等。结核分枝杆菌所导致的结核病仍然是全球人类健康的主要威胁。致病菌中的ClpX蛋白酶在基因的表达调控、致病性以及宿主免疫压力耐受中都具有非常重要的功能。本文总结了ClpX蛋白酶的结构、底物以及所调控的基因；分析了结核分枝杆菌ClpX蛋白酶的进化特征，并探讨了结核分枝杆菌ClpX蛋白酶可能的生理功能和在致病性中的重要作用。

Abstract:

Abstract：ClpX is a member of Hsp100（heat-shock protein） family which is conserved among organisms. Hsp100/ Clp implicates in stress resistance, intracellular protein turn-over, DNA replication and regulation of gene expression. Tuberculosis remains one of the major threats to human health. In pathogens, ClpX protease plays an important role in the gene expression regulation, pathogenesis, and resistance of immune stress. The structure, substrates and target genes of ClpX are summarized in this study. The biological function of M. tuberculosis ClpX, such as gene expression regulation, pathogenesis, intracellular survival and persistence, evolution and structural feature, substrates is the focus of this summary.