益生菌群体感应系统的检测及功能调控

Detection and functional regulation of the probiotic quorum sensing system

摘要:

群体感应(quorum sensing, QS)是一种微生物通过分泌并感知信号分子进行通信，进而调控群体行为的机制，在益生菌生物被膜的形成与肠道定植过程中发挥重要作用。近年来，干预益生菌的QS已成为合成生物学领域的研究热点。本文综述了QS在益生菌中的分布情况，以及基于QS的益生功效干预策略；概述了目前已报道的益生菌QS系统，并对光电化学、色谱-质谱联用等检测益生菌QS的方法进行了总结与分析；重点阐述了干预益生菌QS的主要途径，包括QS激动剂的开发以及相关代谢通路的优化等。本文提出的基于QS的益生菌干预策略，为调控工程益生菌的功效提供了一种新型方案，对益生菌功能食品的开发与改进具有重要意义。

Abstract:

Quorum sensing (QS) is a communication mechanism through which microorganisms secrete and sense signal molecules to regulate mircobial population behaviors. QS plays important roles in biofilm formation and gut colonization of probiotics. In recent years, interfering with the QS of probiotics has become a trending research field of synthetic biology. In this review, we summarize the distribution of QS systems in probiotics and highlights interfering strategies designed to regulate probiotic functions. We summary currently identified QS systems in probiotics and their detection methods, such as photoelectrochemical assays and chromatography-mass spectrometry techniques. Meanwhile, this review outlines the QS interfering approaches for probiotics, including the use of QS agonists and the optimization of related metabolic pathways. Finally, the probiotic intervention strategy targeting QS is proposed in this paper, providing a novel approach for regulating the efficacy of engineered probiotics, which is of great significance for the development and improvement of probiotic functional foods.