活体生物发光成像技术及其在病毒感染研究中的应用

Bioluminescence in-vivo imaging technology and its application in the study of viral infection－A review

摘要:

摘要：生物发光是动物活体光学成像技术之一，因其反应灵敏、操作简单、数据精确，而被广泛地应用于生命科学研究多个领域，观测活体动物体内病毒复制、肿瘤生长等生命过程。生物发光技术采用荧光素酶基因标记细胞或病毒，与外源注射的底物荧光素发生反应，在冷CCD成像系统下显像并进行数据记录、分析。本文简要介绍活体生物发光成像这一新技术的原理，综述了其在发现病毒新的复制位点、研究干扰素及药物对病毒的抑制作用、展示病毒潜伏感染与再激活的历程等方面的应用，并结合自己工作对该技术及其发展前景进行评述。

Abstract:

Abstract: Bioluminescence imaging is one of the bio-optical imaging techniques which report definite biological event in living animals with genetic modification. With high sensitivity, simple operation and high precision, it is particularly applied in observing vital processes like viral infection and tumor growth in vivo. We summarize the principle of bioluminescence imaging, introduce its application in finding virus replication site, study of interferon（IFN） inhibiting-virus effect and real-time visualization of viral latent infection and reactivation, and preview the trend of bioluminescence imaging technological development.