1,3-丙二醇产生菌基因组重排育种

Screening and breeding high 1,3-propanediol producing strains by genome shuffling

摘要:

摘要：【目的】 本文以肺炎克雷伯氏杆菌为研究对象，利用基因组重排技术，提高其对发酵体系中主要产物的耐受性，获得1,3-丙二醇高产菌。【方法】 以含预处理后的出发菌株的补料发酵终点液的96孔板为筛选方法，利用基因组重排技术育种。【结果】 筛选到的5株高产菌株(LSG1, LSG2, LSG4, LSG5, LSG6)，在3升罐批次发酵中的1,3-丙二醇浓度较亲本分别提高了17.0%，19.0%，12.9%，23.9%和18.0%；甘油到1,3-丙二醇的转化率提高了17.7%，20.0%，13.3%，24.4

关键词:关键词：1,3-丙二醇，肺炎克雷伯氏杆菌，基因组重排，原生质体，补料发酵终点液

Abstract:

Abstract: [Objective] To improve the tolerance of main metabolites, we used genome shuffling to achieve high 1,3-propanediol producing mutants. [Methods] Based on 96 deep-well palates containing prepared ended fed-batch broth as an efficient selection method, genome shuffling has been applied in strain improvement. [Results] Five high producers were obtained after genome shuffling (LSG1, LSG2, LSG4, LSG5 and LSG6). During batch fermentation (3 L), the 1,3-propanediol production of the five mutants were improved 17.0%，19.0%，12.9%，23.9% and18.0%, compared with the parent strain; the conservations from glycerol were improved 17.7%，20.0%，13.3%，24.4% and 17.7%. [Conclusion] Genome shuffling was an efficient approach for strain improvement, and 96 deep-well palates containing fed-batch broth has been demonstrated as an efficient selection approach.

Key words:Keywords: 1,3-propanediol, Klebsiella pneumoniae, genome shuffling