呼吸抑制在二氧化氯杀菌中的作用

Bactericidal effects of chlorine dioxide by respiratory inhibition

摘要:

摘要: 【目的】二氧化氯是一种高效低毒的食品和饮用水消毒剂，其杀菌机理至今还不甚明了。本文的目的是研究二氧化氯对白色念珠菌呼吸功能的抑制作用及其与杀菌效应之间的对应性。【方法】超显微结构观察、流式细胞仪检测、耗氧率的测定以及平板培养。【结果】二氧化氯对白色念珠菌的线粒体的结构和外形没有明显的损伤作用，但是线粒体的跨膜电位会随着二氧化氯剂量的增加而逐渐崩溃; 有氧呼吸的抑制程度与菌体的死亡率保持正相关但并不相等，各种作用条件下呼吸的抑制率始终显著低于菌体死亡率; 二氧化氯作用后用厌氧培养和好氧培养两种方法所检测的死亡率没有明显区别。【结论】实验结果说明了二氧化氯对真核细胞的细胞器有明显的损伤作用并与死亡率呈正相关，但呼吸抑制可能不是细菌死亡的首要原因。

Abstract:

Abstract: ［Objective］Chlorine dioxide ( ClO2) is a highly effective disinfectant for food and potable water treatment．However，knowledge on its action mechanism remains unexplored． The present study aims to determine the role of respiratory inhibition in the bactericidal effects of ClO2.[Methods] Transmission electron microscopy was used to observe the ultra structural alteration of the mitochondrion． Fluorescence-based flow cytometry analysis was employed to determine the disruption of mitochondrion membrane potential． Respiratory inhibition was detected by measuring the oxygen consumption． The results obtained were compared with those of plate counting． ［Results］ No visible physiological alteration in the shapes and structures of the mitochondria was found． The rate of collapse in mitochondrial membrane potential increased with the death rate，but the respiratory inhibition rates were always significantly lower than the death rates． The death rates detected by the aerobic and anaerobic methods did not differ significantly． ［Conclusion］ ClO2-induced damages to the mitochondria were positive correlated with the death rates，but respiratory inhibition was not the primary target site for cell killing．