水稻条斑病菌胞外多糖相关基因的鉴定
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国家自然科学基金(31071656;31000071);公益性行业(农业)科研专项(NYHYZX07-056)


Identification of extracellular polysaccharide-associated genes in Xanthomonas oryzae pv.oryzicola
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Supported by the Natural Science Foundation of China(30710103902 ,31000071) and by the Ministry of Agriculture of China (NYHYZX07-056)

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    摘要:

    摘要:【目的】前期研究中从Tn5 转座子插入的水稻条斑病菌突变体库中获得了17 个胞外多糖改变的突变体。【方法】本文对这些突变体的Tn5 插入位点和基因类型进行了鉴定。【结果】结果显示,胞外多糖减少的11 个突变体中多数为Tn5 插入在已知的gum、xan 和wxoc 基因簇上,Xoryp_4217、Xoryp_2488 和Xoryp_0918为未知的与胞外多糖产生有关的基因,属首次报道;6 个胞外多糖增多的突变体中,fimO、pilY 和xopQ 与胞外多糖产生有关,但在水稻条斑病菌中未见报道;Xoryp2392、Xoryp_4221 和Xoryp_3511 为首次鉴定,其中Xoryp_3511 仅在水稻黄单胞菌中存在。毒性测定结果显示,胞外多糖减少的突变体在水稻上的毒性变弱,而胞外多糖增加的突变体在水稻上的毒性没有显著变化。【结论】这些结果为进一步分析水稻条斑病菌胞外多糖代谢途径以及与水稻的互作关系奠定了基础。

    Abstract:

    Abstract:[Objective] Previously,seventeen extracellular polysaccharide-associated mutants of Xanthomonas oryzae pv.oryzicola (Xoc) were acquired from our randomly Tn5-inserted mutant library.To know the Tn5-inserted genes of these mutants and their contribution to EPS production and virulence in rice,[Methods] in this study,we first identified and characterized the Tn5-targeted genes in these mutants and then inoculated them in susceptible rice for virulence assessment.[Results] Tn5 transposon was inserted in genes of the gum,xan and wxoc clusters in the majority of EPSreduced mutants. Of the EPS-reduced mutants,three were due to the Tn5 insertion in Xoryp _4217,Xoryp _2488 and Xoryp_0918 genes,respectively. In six EPS-increased mutants,three were in mutagenesis in fimO,pilY and xopQ genes,respectively,resulting in higher EPS production than the wild-type strain RS105. Other three were because of the mutation in Xoryp2392,Xoryp_4221 and Xoryp_3511 genes. Interestingly,XocORF-3511 only exists in X. oryzae but not in other Xanthomomas species. Virulence assays in rice showed that the less EPS production by the mutant,the weaker the virulence in rice. However,those mutants in higher EPS production did not increase virulence significantly in rice compared to that by the wild-type strain. [Conclusion]Our findings will help further understand the metabolic pathways for EPS synthesis in Xoc and the specific roles of EPS-associated genes in Xoc-rice interactions.

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周丹,邹丽芳,邹华松,陈功友. 水稻条斑病菌胞外多糖相关基因的鉴定. 微生物学报, 2011, 51(10): 1334-1341

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  • 收稿日期:2011-03-19
  • 最后修改日期:2011-05-10
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