封闭循环养殖系统中β变形菌亚纲氨氧化细菌的引物特异性

Comparing the primer specificity for betaproteobacterial ammonia-oxidizing bacteria in recirculation aquaculture systems

摘要:

摘要:【目的】为探讨4 对不同的引物对封闭循环养殖系统生物膜中β 变形菌亚纲氨氧化细菌(β-AOB) 的特异性差异。【方法】采用16S rDNA 文库克隆技术对β-AOB 的多样性进行了分析。【结果】以引物CTO189f/CTO654r 扩增构建的文库中所含β-AOB 比例最高，可达67. 3%。不同封闭循环养殖系统的生物膜对引物的扩增效率有明显的影响，其中以养殖尼罗罗非鱼的封闭循环养殖系统生物膜为DNA 来源的，引物均有较高的扩增效率。【结论】针对封闭循环养殖系统生物膜中的β-AOB，特异性最高的是CTO189f /CTO654r 引物。

Abstract:

Abstract:［Objective］ To analyze the difference of specificity of four primers for betaproteobacterial ammonia-oxidizing bacteria (β-AOB) 16S rDNA gene from the biofilm of closed recirculation aquaculture systems．［Methods］We used 16S rDNA clone libraries to describe the β-AOB diversity． ［Results］ CTO189f /CTO654r produced the highest frequency of β-AOB-like sequences (67.3%)．The amplification performance of primer was noticeably influenced by the biofilm samples． Hereinto，the biofilm of closed recirculation aquaculture systems of Tilapia nilotica resulted in the higher amplification performance of primers．［Conclusion］ CTO189f /CTO654r exhibited the highest specific for β-AOB 16S rDNA gene from the biofilm of closed recirculation aquaculture systems．