黄曲霉毒素对费氏弧菌发光的影响

Influence of aflatoxin on Vibrio fischeri luminescence

摘要:

摘要:【目的】探讨黄曲霉毒素对一种发光细菌———费氏弧菌发光的抑制效应。【方法】黄曲霉毒素或产黄曲霉毒素的菌株培养液对费氏弧菌进行处理后，利用多功能酶标仪检测费氏弧菌的发光强度，研究黄曲霉毒素对费氏弧菌发光的影响。【结果】黄曲霉毒素浓度的对数值与费氏弧菌发光的抑制率呈线性关系，依据所得的回归方程可以快速准确地检测不同微生物产毒素的情况:6 株不同来源的黄曲霉菌株均能够产毒素，以黄曲霉毒素含量表示的毒素量在14.94－46.45mg/L 之间，1株米曲霉不产毒素。【结论】费氏弧菌发光强度的改变可以较准确地反映微生物产毒素的能力，尤其是微生物产黄曲霉毒素的能力，为在工农业生产中快速检测黄曲霉毒素提供了新的线索，有望发展成为一种检测黄曲霉毒素的新技术。

Abstract:

Abstract: ［Objective］ In the present study，we aim to evaluate the inhibitory effect of aflatoxin on Vibrio fischeri luminescence．［Methods］ V． fischeri culture is treated with aflatoxin or the culture broth of aflatoxin-producing strains，and the luminescence intensity of V． fischeri is detected to analyze the influence of aflatoxin on V． fischeri．［Results］The logarithmic value of aflatoxin concentration and the decrease ratio of V． fischeri luminescence is in a linear relationship．Based on the regression equation between aflatoxin concentration and luminescence decrease of V． fischeri，the toxinproducing status of different microbes can be detected quickly and exactly: all of six tested Aspergillus flavus strains show toxigenicity to V．fischeri，and their toxin yield reached 14. 94 mg/L－46.45 mg/L (represented by aflatoxin concentration)，while the tested Aspergillus oryzae shows no toxigenicity． ［Conclusion］ The above data showed that the luminescence change of V． fischeri could exactly reflect the capability of various microbes to produce toxin (especially aflatoxin)，which provided a new clue for rapid detection of aflatoxin in industrial and agricultural production and could bedeveloped as a potential method for aflatoxin assay．