酿酒酵母生产羧酸的代谢工程策略

Metabolic engineering strategies for carboxylic acids production by Saccharomyces cerevisiae—A review

摘要:

摘要:羧酸广泛地应用于食品、医药和化工等行业，具有广阔的市场前景。作为真核模式微生物，酿酒酵母作为代谢工程平台用来生产有机酸具有明显优势。本文论述了酿酒酵母生产重要羧酸的策略:首先构建一条能够和糖酵解途径相连接的高效的重要羧酸积累途径，进而探讨如何将碳代谢流由乙醇转向目的产物，在此基础上研究有机酸的转运及涉及到的能量问题。最后，对当前研究存在的问题进行了分析，并对未来研究方向进行了展望。

关键词:关键词: 酿酒酵母，羧酸，代谢工程，系统生物学

Abstract:

Abstract:Carboxylatic acids have been widely used in food，pharmaceutical and chemical industries．As a eukaryotic model organism， Saccharomyces cerevisiae is thought as cell factory to produce organic acids through manipulating metabolic pathway．In this review，we addressed the metabolic engineering strategies to construct a high titer route converting pyruvate to target carboxylate，and to explore how to divert the carbon flux to desired product from ethanol．Furthermore，we also discussed the mechanisms for carboxylate transport and energy involved in． Finally，the relevant strategies for development in future are proposed．

Key words:Keywords: Saccharomyces cerevisiae，carboxylatic acids，metabolic engineering，systems biology