陕西省不同种植区长梗绞股蓝土壤理化性质和根部土壤细菌群落结构分析

Soil physicochemical properties and root soil bacterial composition of Gynostemma longipes in different planting regions of Shaanxi Province

摘要:

目的 比较不同种植地长梗绞股蓝根际和非根际土壤细菌多样性和群落组成差异，结合环境因子关联分析，揭示影响长梗绞股蓝土壤细菌群落的关键因子，为长梗绞股蓝的栽培引种提供参考，也为进一步探究不同产区长梗绞股蓝根际微生物与化学成分含量的关系奠定基础。方法 基于高通量测序技术和土壤理化性质分析，比较不同种植地长梗绞股蓝土壤细菌群落多样性和组成差异，并揭示影响细菌群落的关键环境因子。结果 共获得97 085个细菌扩增子序列变体(amplicon sequence variants, ASVs)，长梗绞股蓝土壤细菌群落结构在不同种植地间具有显著差异(R=0.562，P=0.001)，在根际和非根际土壤中无显著差异。变形菌门(Proteobacteria，27.40%-36.67%)和酸杆菌门(Acidobacteriota，15.60%-22.19%)为长梗绞股蓝土壤细菌中的优势菌门。土壤pH、有效磷(available phosphorus, AP)、速效钾(available potassium, AK)、有机质(soil organic matter, SOM)和碱解氮(alkali-hydrolyzable nitrogen, AN)是影响长梗绞股蓝土壤细菌群落结构的关键土壤环境因子。结论 基于本研究的样本分析，不同产地长梗绞股蓝细菌群落多样性和组成差异显著且与土壤理化性质密切相关。本研究为长梗绞股蓝的引种栽培提供了一定的参考，也为进一步探究绞股蓝土壤微生物与次生代谢产物积累的关系奠定了基础。

关键词:长梗绞股蓝;根部土壤;细菌群落;理化性质

Abstract:

Objective To compare the bacterial diversity and community composition between the rhizosphere and non-rhizosphere soil of Gynostemma longipes in different planting regions and reveal the key environmental factors by correlating the bacterial community composition with soil physicochemical properties. The findings are expected to provide a reference for the cultivation and introduction of this plant and lay a basis for exploring the relationship between rhizosphere microorganisms and the chemical component content of G. longipes in different planting regions.Methods High-throughput sequencing and soil physicochemical property measurement were employed to compare the bacterial diversity and community composition of G. longipes in different planting regions and reveal the key environmental factors influencing the bacterial community.Results A total of 97 085 bacterial amplicon sequence variants (ASVs) were obtained. The bacterial community composition in G. longipes soil showed significant differences among different planting regions (R=0.562, P=0.001) but no significant differences between rhizosphere and non-rhizosphere soil. Proteobacteria (27.40%?36.67%) and Acidobacteriota (15.60%?22.19%) were the dominant bacterial phyla. Soil pH, available phosphorus, available potassium, soil organic matter, and alkali-hydrolyzable nitrogen were identified as key environmental factors influencing the bacterial community composition in G. longipes soil.Conclusion Based on the sample analysis in this study, the bacterial community diversity and composition of G. longipes varied significantly aross different locations and were closely associated with soil physicochemical properties. This study provides a reference for the cultivation and introduction of G. longipes and gives insights into the relationship between soil microorganisms and secondary metabolite accumulation of G. longipes.

Key words:Gynostemma longipes;rhizosphere;bacterial community;physicochemical properties