2025年7月18日 周五
首页 > 过刊浏览>2025年第65卷第6期 >2463-2478. DOI:10.13343/j.cnki.wsxb.20240797
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陕西省不同种植区长梗绞股蓝土壤理化性质和根部土壤细菌群落结构分析
作者:
作者单位:

1.中国医学科学院北京协和医学院药用植物研究所，道地药材品质保障与资源持续利用全国重点实验室，北京;2.山东第二医科大学 药学院，山东 潍坊

作者简介:

冷春燕：样品处理、论文撰写；尹一飞：协助样品处理和实验操作；侯梦妍：数据收集和处理；于晶：数据收集和处理；李绕静：文章修改；邢咏梅：数据核查并参与论文讨论；陈娟：研究构思和实验设计并修改讨论论文；郭宝林：实验材料采集、研究构思并参与论文讨论。

基金项目:

陕西省2024年重点研发计划(2024SF-GJHX-10)；中国医学科学院医学与健康科技创新工程(重大协同创新项目) (2021-I2M-1-032)；山东省泰山学者青年专家项目(Tsqn202211233)


Soil physicochemical properties and root soil bacterial composition of Gynostemma longipes in different planting regions of Shaanxi Province
Author:
Affiliation:

1.State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China;2.School of Pharmacy, Shandong Second Medical University, Weifang, Shandong, China

Fund Project:

This work was supported by the Key Research and Development Project of Shaanxi Province in 2024 (2024SF-GJHX-10), the CAMS Innovation Fund for Medical Sciences (CIFMS) (2021-I2M-1-032), and the Special Fund for Taishan Scholar Project (Tsqn202211233).

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    摘要:

    目的 比较不同种植地长梗绞股蓝根际和非根际土壤细菌多样性和群落组成差异，结合环境因子关联分析，揭示影响长梗绞股蓝土壤细菌群落的关键因子，为长梗绞股蓝的栽培引种提供参考，也为进一步探究不同产区长梗绞股蓝根际微生物与化学成分含量的关系奠定基础。方法 基于高通量测序技术和土壤理化性质分析，比较不同种植地长梗绞股蓝土壤细菌群落多样性和组成差异，并揭示影响细菌群落的关键环境因子。结果 共获得97 085个细菌扩增子序列变体(amplicon sequence variants, ASVs)，长梗绞股蓝土壤细菌群落结构在不同种植地间具有显著差异(R=0.562，P=0.001)，在根际和非根际土壤中无显著差异。变形菌门(Proteobacteria，27.40%-36.67%)和酸杆菌门(Acidobacteriota，15.60%-22.19%)为长梗绞股蓝土壤细菌中的优势菌门。土壤pH、有效磷(available phosphorus, AP)、速效钾(available potassium, AK)、有机质(soil organic matter, SOM)和碱解氮(alkali-hydrolyzable nitrogen, AN)是影响长梗绞股蓝土壤细菌群落结构的关键土壤环境因子。结论 基于本研究的样本分析，不同产地长梗绞股蓝细菌群落多样性和组成差异显著且与土壤理化性质密切相关。本研究为长梗绞股蓝的引种栽培提供了一定的参考，也为进一步探究绞股蓝土壤微生物与次生代谢产物积累的关系奠定了基础。

    Abstract:

    Objective To compare the bacterial diversity and community composition between the rhizosphere and non-rhizosphere soil of Gynostemma longipes in different planting regions and reveal the key environmental factors by correlating the bacterial community composition with soil physicochemical properties. The findings are expected to provide a reference for the cultivation and introduction of this plant and lay a basis for exploring the relationship between rhizosphere microorganisms and the chemical component content of G. longipes in different planting regions.Methods High-throughput sequencing and soil physicochemical property measurement were employed to compare the bacterial diversity and community composition of G. longipes in different planting regions and reveal the key environmental factors influencing the bacterial community.Results A total of 97 085 bacterial amplicon sequence variants (ASVs) were obtained. The bacterial community composition in G. longipes soil showed significant differences among different planting regions (R=0.562, P=0.001) but no significant differences between rhizosphere and non-rhizosphere soil. Proteobacteria (27.40%?36.67%) and Acidobacteriota (15.60%?22.19%) were the dominant bacterial phyla. Soil pH, available phosphorus, available potassium, soil organic matter, and alkali-hydrolyzable nitrogen were identified as key environmental factors influencing the bacterial community composition in G. longipes soil.Conclusion Based on the sample analysis in this study, the bacterial community diversity and composition of G. longipes varied significantly aross different locations and were closely associated with soil physicochemical properties. This study provides a reference for the cultivation and introduction of G. longipes and gives insights into the relationship between soil microorganisms and secondary metabolite accumulation of G. longipes.

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冷春燕,尹一飞,侯梦妍,于晶,李绕静,邢咏梅,陈娟,郭宝林. 陕西省不同种植区长梗绞股蓝土壤理化性质和根部土壤细菌群落结构分析[J]. 微生物学报, 2025, 65(6): 2463-2478

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  • 收稿日期:2024-12-10
  • 在线发布日期: 2025-06-05
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