四川冬菜中细菌群落组成及多样性

Bacterial biodiversity in Dongcai,a traditional pickled mustard product in Sichuan Province，China

摘要:

摘要:【目的】了解腌制4 年的四川南充冬菜中细菌群落组成及多样性。【方法】通过16S rDNA 多样性分析样品细菌落组成;采用16S rDNA-RFLP 方法分析从样品中分离出的纯培养细菌。【结果】16S rDNA 多样性分析结果表明，样品中细菌主要属于变形杆菌门(Proteobacteria)和厚壁菌门(Firmicutes)，分别占克隆文库的87.9%、7.1%，其中包括Virgibacillus kekensis，Marinococcus albus，Salinicoccus sp.，Lactobacillus halophilus和Halomonas 等中度嗜盐菌，仅有5% 属于放线菌门(Actinobacteria)。通过纯培养方法从冬菜中分离到35 株菌，16S rDNA-RFLP 分析结果表明，34 株属于厚壁菌门(Firmicutes)，包括Virgibacillus，Bacillus megaterium 和Gracilibacillus saliphilus等中度嗜盐菌，1株属于放线菌门(Actinobacteria)。【结论】冬菜中细菌群落多样性较低，以中度嗜盐菌为主。

Abstract:

Abstract:［Objective］To investigate the bacteria community and biodiversity of four-years pickled Yanshan Dongcai．［Methods］We studied the bacterial communities of Dongcai by 16S rDNA diversity analysis and the cultured species isolated from Dongcai sample by Restriction Fragment Length Polymorphism (RFLP) and 16S rRNA gene sequence analysis．［Results］ The 16S rDNA diversity showed that the bacteria belonged to the phyla Proteobacteria (87.9%) and Firmicutes (7.1%)，including many moderately halophilic bacteria such as Virgibacillus kekensis，Marinococcus albus，Salinicoccus sp.，Lactobacillus halophilus and Halomonas． Only 5% of clone sequences belonged to the phylum Actinobacteria． Thirty-five strains were isolated from Dongcai sample，and 16S rDNA-RFLP analysis indicated that 34 isolates affiliated with the phylum Firmicutes，including Virgibacillus，Bacillus megaterium and Gracilibacillus saliphilus which were moderately halophilic bacteria，but only one isolate belonged to the phylum Actinobacteria． ［Conclusion］The bacterial diversity is low in Dongcai，dominated by moderately halophilic bacteria.