炭疽杆菌在家兔盲肠结扎模型内外培养的蛋白表达差异

Differential expression proteins of Bacillus anthracis cultured in rabbit’s intestinal tract model

摘要:

摘要：【目的】建立家兔盲肠结扎模型，研究炭疽杆菌在家兔体内外不同培养条件下的蛋白表达差异。【方法】本实验通过进行家兔盲肠结扎模型对炭疽杆菌进行体内外培养，用不同方法提取胞外蛋白、细胞壁蛋白及全菌体蛋白，并经双向电泳分离和质谱鉴定。【结果】送检144个蛋白点，检出124个，其中包括上清蛋白19个，细胞壁蛋白29个，全菌体蛋白76个。【结论】经分析发现，与合成代谢相关的蛋白在体内主要呈下调趋势，包括多种氨基酰－tRNA合成酶、长链脂肪酸CoA连接酶等；在体内表达上调的蛋白则具有多种功能，其中包括分子伴侣DnaK、超氧化物歧化酶SodA、 S-层蛋白等。

Abstract:

Abstract：[Objective] The rabbit’s intestinal tract model was constructed to analyze the different expression proteins of Bacillus anthracis in vitro and in vivo. [Methods] We used the rabbit’s intestinal tract model to culture the Bacillus anthracis, using different methods to extract the extracellular proteins, cell wall proteins and whole cellular proteins from the in vivo and in vitro samples. Those proteins were separated by two－dimensional gel electrophoresis and analyzed by mass spectrum. [Results] We had been distinguished about 144 differential expression protein spots between in vivo and in vitro samples, and 124 spots were identified, including 19 supernatant proteins, 29 cell wall proteins and 78 whole cellular proteins. [Conclusion] Those proteins which related to the anabolic metabolism were down-regulated in vivo, including threonyl－tRNA synthetase, prolyl-tRNA synthetase, long-chain-fatty-acid-CoA ligase. There were also many up-regulated proteins in vivo, such as DnaK, SodA and S-layer proteins.