芳香聚酮早期后修饰环化酶的结构分类和功能分析

Structural classification and functional analysis of aromatic polyketide cyclases－A review

摘要:

摘要：聚酮是一类结构和生物活性多样的天然产物，根据结构特点可以分为芳香聚酮和复合聚酮两大类。芳香聚酮环化酶是芳香聚酮生物合成过程中一种非常重要的早期后修饰酶，是决定芳香聚酮骨架结构的主要影响因素。根据序列和结构的相似性，芳香聚酮环化酶可以分为不同的种类。本文主要对其中3类芳香聚酮环化酶结构和功能进行了简要总结，从晶体结构、催化反应和催化机制等方面对它们进行了分类描述和功能分析，并结合自己实验室工作介绍了杰多霉素B环化酶催化机制的研究方法。

Abstract:

Abstract: Polyketides represent a large group of natural products with diverse structures and biological activities, which could be divided into two structural types: aromatic polyketides and complex polyketides. Cyclases are key enzymes involved in the early tailoring reactions after polyketide chain synthesis; they act in concert with other early tailoring enzymes to control the folding and cyclization pattern of aromatic polyketides. Therefore, they are the key determinants of the final polyketide scaffold. Here, we classified cyclases involved in aromatic polyketide biosynthesis based on their sequences and structures. The reactions catalyzed by these cyclases and the possible catalytic mechanisms were described. We also described the research progress on the characterization of cyclases in jadomycin B biosynthesis.