一株新的溶磷棘孢青霉菌Z32的分离、鉴定及其土壤定殖与溶磷特性

Isolation and identification of a novel phosphate-dissolving strain Penicillium aculeatum Z32 and its colonization and phosphate-dissolving characteristics in soil

摘要:

摘要：【目的】从植物根部土壤中分离到一株高效溶磷真菌Z32，进行了分类学鉴定和土壤定殖与溶磷特性的初步研究。为溶磷微生物的应用提供新的菌株。【方法】通过形态特征、培养特征和ITS rDNA序列分析方法进行菌株鉴定。通过菌株Z32在土豆液体培养基培养过程中培养液pH的变化确定溶磷菌株的溶磷能力。利用菌株土培试验，进行菌株的土壤定殖和土壤中不同形态无机磷转化试验。【结果】菌株Z32鉴定为棘孢青霉菌（Penicillium aculeatum）。菌株Z32能在4 d内完全溶解固体培养基中的磷酸三钙，18 h内将土豆液体培养基的pH值从7.0降低到1.5左右。菌株Z32在20℃时的定殖效果最好，21 d时，菌数达到起始的9.83倍，而且能够保持到49 d不消亡。在20℃时，添加菌株Z32的土培实验在21 d时，土壤中Ca8H2(PO4)6?5H2O、AlPO4和FePO4等难溶无机磷向可溶性的CaHPO4转化，CaHPO4含量增加了58.83%。49 d时，土壤中的Ca8H2(PO4)6?5H2O、AlPO4和和FePO4被转化后，没有随着微生物的减少而完全被固定。【结论】筛选到一株新的溶磷菌株Z32在土壤中很好定殖，能够转化土壤中多种形态的难溶无机磷为可溶性的CaHPO4，显著增加土壤中CaHPO4的含量。同时阻滞了Ca8H2(PO4)6?5H2O、AlPO4和和FePO4等难溶无机磷的固定。

Abstract:

Abstract: 【Objective】To obtain new strains that can dissolving phosphate, we screened and identified a new strain Z32 capabile of phosphate dissolving from soil samples. We studied the colonization and phosphate-dissolving characteristics of Z32 in soil.【Methods】Morphological, growth on various media, and ITS rDNA sequences homology analysis were performed to identify the strain Z32. We conducted a soil experiment in pot to investigate the colonization and the capbility of the different forms of inorganic phosphorus transformation of the strain Z32.【Results】The strain Z32 was identified as Penicillium aculeatum. Z32 dissolved all Ca3(PO4)2 in solid culture for 4 days and decreased the pH from 7.0 to 1.5 in potato liquid culture for 18 hours. Z32 presented high colonization ability in soil and the optimum colonization temperature was 20℃. The number of Z32 increased 9.83 times in 21 days and kept alive in 49 days at the optimum temperature. Z32 promoted the transformation of insoluble phosphate like Ca8H2(PO4)6?5H2O, AlPO4 and FePO4 to soluble CaHPO4 and the CaHPO4 content increased by 58.83% in soil compared to CK at the 21st d at 20℃. Conclusion】 A new strain Z32 with high performance of phosphate dissolving had colonized well in soil. Z32 showed high phosphate-dissolving ability for different kinds of inorganic phosphorus in soil. At the same time, Z32 inhibited the transformation of CaHPO4 to insoluble phosphate.