M41型A群链球菌的Ⅰ型胶原样蛋白与人低密度脂蛋白的相互作用

Scl1 of M41-type group A streptococcus binds low-density lipoprotein

摘要:

摘要：【目的】检测M41型A群链球菌（GAS）ATCC12373中Ⅰ型胶原样蛋白（Scl1）与人低密度脂蛋白（LDL）的相互作用。【方法】克隆了M41型GAS ATCC12373的Ⅰ型胶原样蛋白（Scl1）及其V区（Scl1-V）基因，并表达、纯化重组蛋白rScl1 (C176)和rScl1-V (C176V)。通过重组蛋白与人血浆的亲和色谱层析、Western blot及酶联免疫吸附试验（ELISA）检测C176、C176V与LDL的相互作用；通过GAS与LDL的ELISA试验和人血浆与GAS的共孵育试

Abstract:

Abstract: [Objective] To study the interaction of streptococcal collagen-like protein 1 (Scl1) of M41-type group A streptococcus (GAS) ATCC12373 with low-density lipoprotein (LDL). [Methods] We cloned, expressed and purified the recombinant proteins rScl1 and its V region rScl1-V, designated as C176 and C176V, derived from Scl1.41 of M41-type GAS. The binding of rScl1 to LDL was detected with affinity chromatography-binding assay, Western blot analysis and enzyme-linked immunosorbent assay (ELISA), and whole cell binding assays were used to detect the interactions of whole GAS cells with LDL. [Results] The results demonstrated that C176 and C176V could specifically bind purified LDL, and M41-type GAS cells expressing native Scl1 could bind LDL whereas M6-type GAS could not. [Conclusion] The Scl1 of M41-type GAS specifically binds LDL.