产紫青霉EL-02的分离鉴定及其絮凝活性

Flocculating activity of Penicillium purpurogenum EL-02

摘要:

摘要：【目的】分离鉴定有絮凝活性真菌，同时对其絮凝活性进行初步研究。【方法】采用梯度稀释、平板划线、18S rDNA检测等方法分离鉴定絮凝活性菌株。通过高速离心、超声破碎、乙醇沉淀、定性试验等方法确定絮凝活性物质性质。【结果】从渤海湾海岸土壤样品中分离筛选出一株有较高絮凝活性的真菌，经鉴定为产紫青霉（Penicillium purpurogenum），命名为产紫青霉EL-02（P. purpurogenum EL-02）。超声破碎试验证实其絮凝活性主要存在于发酵上清液。根据絮凝活性曲线，确定4 d为积累絮

Abstract:

Abstract: [Objective] To isolate, identify and characterize a fungus with flocculating activity. [Methods] We used gradient dilution, plaque distribution and 18S rDNA analysis to isolate and identify a fungus with flocculating activity. We used high-speed centrifugation, ultrasonication and qualitative test to determine the nature of flocculating active substances. [Results] We isolated a flocculating active fungi and identified it as Penicillium purpurogenum. Ultrasonication test confirmed that the flocculating activity was primarily in the fermentation supernatant. We explored the flocculating activity curve and found that 4 days was the optimum fermentation time for accumulating flocculating active substances. The strain flocculating activity remains unchanged when pH varied from 2 to 11 and temperature varied from -70℃ to 100℃.We finally identified the flocculating active substances as saccharides. [Conclusion] We isolated a flocculating active strain P. purpurogenum EL-02, and identified its flocculating active products as saccharides.