聚乙二醇作为乙型肝炎病毒DNA疫苗佐剂的体外试验

Effect of polyethylene glycol as adjuvant on hepatitis B virus DNA vaccine in vitro

摘要:

摘要：【目的】研究聚乙二醇作为佐剂是否能够增强HBV DNA疫苗体液和细胞免疫效果。【方法】我们将乙型肝炎病毒(HBV) DNA 疫苗（pVAX-S2）单独或PEG/pVAX-S2免疫C57BL/6小鼠，在最后一次免疫1 d检测抗-HBs IgG、T淋巴细胞增殖、细胞因子表达及体内细胞毒T淋巴细胞杀伤作用（CTL）等免疫学指标。【结果】结果表明，与仅免疫pVAX-S2比，PEG/ pVAX-S2能够增加免疫小鼠抗-HBs IgG水平; 混合免疫组的T淋巴细胞体外经乙肝表面抗原（HBsAg）刺激后，T淋巴细

Abstract:

Abstract: [Objective] To explore whether polyethylene glycol (PEG) as adjuvant could enhance the humoral and cellular immune responses on hepatitis B virus DNA vaccine. [Methods] C57BL/6J mice were immunized with PEG and pVAX-S2 or alone pVAX-S2. After these mice were finally immunized for 14 days, the anti-HBs IgG, T cell proliferation, the expression of cytokines and CTL in vivo were detected. [Results] Compared to mice immunized with alone pVAX-S2, PEG as adjuvant could increase the production of anti-HBs IgG and HBsAg specific T cell proliferation. In addition, the expression of IL-4, IFN-γ in CD4+ T cells and IFN-γ in CD8+ T cells was higher than control groups. The PEG/pVAX-S2 groups could elicit significantly in vivo HBsAg specific CTL responses. [Conclusions] PEG as adjuvant could enhance humoral and cellular immune responses, as well as in vivo CTL activity.