三株高效秸秆纤维素降解真菌的筛选及其降解效果

Screening of three straw-cellulose degrading microorganism

摘要:

摘要：【目的】利用多种筛选方法，获得高效秸秆纤维素降解真菌，并研究其秸秆纤维素的降解能力。【方法】采用滤纸片孔洞法、滤纸条降解法、羧甲基纤维素钠(CMC-Na)水解圈测定法、秸秆失重法、纤维素分解率测定法、胞外酶活测定法等常规秸秆纤维素降解菌的筛选方法。【结果】筛选到3株具有较强纤维素降解能力的真菌菌株，经初步鉴定菌株98MJ为草酸青霉(Penicillium oxalicum)、菌株W3为木霉(Trichoderma sp.)、菌株W4为扩张青霉(Penicillium expansum)。菌株W4具有

Abstract:

Abstract: [Objective] The aim of this study was to screen straw-cellulose degrading microorganisms and to investigate their degradation ability of straw-cellulose. [Methods] The methods used to screen the high effect straw-cellulose degrading microorganism included the traditional isolation methods of straw-cellulose degrading microorganism such as holes observation method on filter paper sheet, disintegration test of filter paper scrip, hydrolysis spot diameter measurement method of CMC-Na, weight lose assay method of straw, measurement method of cellulose decomposition rate, measurement method of extracellular enzyme activity. [Results] We isolated 3 fungi with cellulose degrading ability, of which 98MJ was identified as Penicillium oxalicum, W3 as Trichoderma sp., and W4 as Penicillium expansum. Strain W4 possessed high straw-cellulose degrading ability with straw-cellulose degrading rate of 56.3%, cellulose 59.06%,, hemicellulose 78.75% and lignin 33.79% in 10 days. [Conclusion] Strain W4 was a cellulase-producing strain with broad development potential.