绿僵菌异戊烯基转移酶基因的克隆及表达谱分析

Cloning and expression of prenyl transferase gene of Metarhizium ansopliae

摘要:

摘要：【目的】 克隆绿僵菌异戊烯基转移酶(Mpt)基因，了解该基因的结构和表达特征。【方法】采用SMART(Switching Mechanism At 5' end of RNA Transcript)技术和PCR技术，扩增Mpt基因全长cDNA序列和DNA序列，用qRT-PCR方法分析该基因在绿僵菌不同侵染时期的表达谱。【结果】Mpt基因含2个外显子和1个内含子，CDS区为1026bp（GenBank登录号GU271134），编码341个氨基酸；qRT-PCR分析表明，该基因在绿僵菌侵染的不同时期表达

Abstract:

Abstract: [Objective ] To clone and identify prenyl transferase gene from Metarhizium anisopliae and to understand the gene structure and expression. [Methods ] Using Switching Mechanism At 5' end of RNA Transcript (SMART) method, we isolated the full length cDNA sequence and DNA sequence. ?Then we used quantitative RT-PCR analysis of the gene expression levels at different stages of colonization of host hemolymph by M. anisopliae. [ Results] The Mpt gene had two exons and one intron and the CDS was 1026 bp which encoded a protein with 341 amino acid residues; qRT-PCR analysis showed that the gene expression levels were significantly different, especially highly up-regulated at the late stages. [ Conclusion] The Mpt gene was successfully cloned from M. anisopliae for the first time and the gene had the characteristic of high expression levels at the late stages.