一株分离自高海拔土壤的梭菌的鉴定及代谢产物

Identification and metabolism characterization of a Clostridium lituseburense strain isolated from high- altitude soil

摘要:

摘要：【目的】了解土壤环境中细菌的生理生化特性及代谢产物。【方法】采用厌氧操作技术从西藏纳木错分离到一株厌氧梭状芽孢杆菌P4-1。采用生理生化鉴定结合16S rDNA序列的系统发育学分析确定该菌株的系统发育地位，用气相色谱分析其代谢产物【结果】菌株P4-1为严格厌氧革兰氏阳性菌，菌体大小为0.3 μm×1 μm－3 μm,单生或成对生长，产生芽孢。生长温度范围是13－40 ℃（最适温度37 ℃）;NaCl浓度0%－5 %（最适为0.5%－1%）;pH范围 5.0－10.0（最适生长pH为7.5－8.0）。

Abstract:

Abstract: [Objective] We studied the physiological, biochemical properties and metabolism of Clostridium lituseburense P4-1 from soil in Namucuo. [Methods] We adopted Hungate anaerobic technique to get Strain P4-1 from soil in Namucuo. Through physiological, biochemical and phylogenetic analysis, we identified the strain P4-1. [Results] Cells were Gram-positive and spore-forming. It grew between 13 and 40℃ (optimum at 37℃), between pH value 5.0 and 10.0 (optimum at 7.5－8.0), and with the presence of NaCl between 0%－5%. Strain P4-1 could metabolize many carbon sources including glucose, melibiose and mannitol. Metabolites of glucose were acetate, butyrate, propionate, CO2, and little H2. Based on 16S rDNA studies, strain P4-1 was most close to Clostridium lituseburense DSM 797(M59107) with 98.7% similarity. Strain P4-1 could degrade p-toluene sulfonate. [Conclusion] Strain P4-1 tolerated low temperature, salt and could degrade p-toluene sulfonate. Its metabolites produced by fermentation of glucose could improve the soil micro-environment. It was significant for strain P4-1 to be utilized in the wastewater treatment.