番鸭源H6N6亚型禽流感病毒全基因组的分子特征

Characterizations of avian influenza virus H6N6 subtype isolated from domestic Muscovy duck

摘要:

摘要：【目的】为了丰富水禽源禽流感病毒的分子流行病学资料，明确我国国内首次分离的番鸭源H6N6亚型禽流感（Avian influenza virus, AIV）病毒A/Muscovy Duck/Fujian/FZ01/2008（H6N6）（以下简称MD/FJ/F1/08）全基因组的分子特征，弄清该病毒的遗传进化特点。【方法】对其8个基因片段分别进行扩增和序列测定，并利用分子生物学软件对测序结果进行序列分析。【结果】MD/FJ/F1/08的HA裂解位点附近的氨基酸序列为PSMKVIV↓GL，为非连续的碱性氨

Abstract:

Abstract: [Objective] To enrich the epidemiologic data of the waterfowl origin avian influenza virus (AIV). [Methods] The full genome of A/Muscovy Duck/Fujian/FZ01/2008(H6N6), which was first isolated from domestic Muscovy duck in Fujian Province in southern China at 2008, was cloned and sequenced by reverse transcription polymerase chain reaction (RT-PCR)．[Results]The motif of hemagglutinin (HA) cleavage site was PSMKVIV↓GL, which is a molecular characteristic of low pathogenic AIV, and the intravenoys pathogenicity index (IVPI) was 0.15. The HA gene, NP gene, M gene and PB2 gene nucleotide sequences had similarity highest with A/duck/Kingmen/E322/04(H6N2) at 94.2%, 95.7%, 97.2% and 95.6%, shared the same phylogenetic lineage. The neuraminidase (NA) gene had similarity highest with A/duck/Eastern China/01/2007(H4N6) at 97.1 per cent, and also had 11 continuous amino acids (TNSTTTIINNN) deletion in the NA gene stalk region, which was first reported had deletion 11 amino acids in N6 subtype AIV NA genes’ ORF. The NA gene had familiar phylogenetic relationship with H4N6 subtype AIV. The NS gene、PB1 gene and the PA gene had genetically close relationships with the H5N1 high pathogenic AIV strains isolated in Hong Kong at 1997. The eight genes also showed any immediate ancestor with other H6N6 subtype avian influenza viruses isolated in North America. [Conclusion] These data showed that A/Muscovy Duck/Fujian/FZ01/2008 (H6N6) was possibly a reassortant virus derived from H6N2 subtype、H4N6 subtype and H5N1 subtype AIV.