聚合酶链反应-酶切分型鉴定广州地区环境水源军团菌

Identification of Legionella isolates from environmental water by using PCR combined with enzymatic digestion analysis

摘要:

摘要：【目的】探讨聚合酶链反应-酶切分型在快速鉴定环境水源军团菌方面的应用价值，并了解广州地区环境水源军团菌的分布状况。【方法】对广州地区采集的44份环境水样，作军团菌分离培养，再对分离菌株进行16S rDNA PCR-酶切分型鉴定、16S rDNA基因测序和mip基因测序鉴定。【结果】在广州地区环境水源分离的112株军团菌，经聚合酶链反应-酶切分型鉴定、16S rDNA基因测序和mip基因测序鉴定，检出嗜肺军团菌66株，非嗜肺军团菌46株，其中菲氏军团菌20株，戈氏军团菌17株，橡树岭军团菌7株，长滩军团菌2株。【结论】聚合酶链反应-酶切分型检测环境水源军团菌是一种简便、快速、特异的鉴定方法；在广州地区环境水源中普遍存在军团菌，主要是嗜肺军团菌，其次是菲氏军团菌，戈氏军团菌，橡树岭军团菌和长滩军团菌。

Abstract:

Abstract: [Objective] To evaluate polymerase chain reaction (PCR) combined with enzymatic digestion for identification of Legionella, and distribution of Legionella in environmental water systems in Guangzhou. [Methods] Forty-four water samples collected in Guangzhou were cultivated for Legionella were identified by PCR-enzymatic digestion, 16S rDNA and mip gene sequencing analysis. [Results] Sixty-six strains of Legionella pneumophila and 46 Non-L. pneumophila were identified by PCR-enzymatic digestion and sequencing analysis. Forty-six strains of Non-L. pneumophila included 20 strains of L. feeilei, 17 L. gormanii, 7 L. oakridgensis and 2 L. longbeachae. [Conclusion] PCR combined with enzymatic digestion is a simple, rapid, and specific method for the identification of Legionella. L. pneumophila was distributed widely, followed by L. feeilei, L. gormanii, L. oakridgensis and L. longbeachae, in environmental water in Guangzhou area.