水稻白叶枯病菌EAL结构域蛋白VieAxoo基因缺失突变和功能分析

Gene deletion and functional analysis of the EAL domain protein VieAxoo in Xanthomonas oryzae pv. Oryzae

摘要:

摘要：【目的】旨在揭示水稻白叶枯病菌(Xanthomonas oryzae pv. oryzae, 简称Xoo) 环鸟苷二磷酸（c-di-GMP）信号蛋白VieAxoo的生物学功能。【方法】本研究通过标记置换法对vieAxoo基因(PXO_04753)进行了缺失突变研究，采用表性测定进行了部分功能鉴定。【结果】从野生型菌株PXO99A中克隆的vieAxoo基因序列与其它病原黄单胞菌的同源序列高度保守。VieAxoo具有参与c-di-GMP降解的磷酸二酯酶（PDE）EAL结构域和磷酸信号识别受体REC结构域

关键词:关键词：水稻白叶枯病菌;环鸟苷二磷酸;vieAxoo;缺失突变;功能鉴定

Abstract:

Abstract: [Objective]To better reveal the functions of key members involved in cyclic di-GMP signal metabolism pathways in the rice bacterial blight pathogen rice Xanthomonas oryzae pv. oryzae (Xoo). [Methods] vieAxoo (PXO_04753), a gene putatively encoding the EAL domain proteins was investigated by gene deletion mutation using the marker exchange, complementation and phenotypic analysis. [Result] The sequence of vieAxoo cloned from genomic DNA of the wild-type strain PXO99A was found to be highly conserved in plant-pathogenic Xanthomonas spp. VieAxoo was structurally featured with EAL and REC domains. No significant changes in virulence to rice, EPS production and flagellar motility were found in △vieAxoo compared to PXO99A, whereas remarkable changes in induction of hypersensitive responses (HR) in tobacco and biofilm formation were observed. [Conclusion] VieAxoo might function as an important regulator in cyclic di-GMP signaling and regulation of bacterial induction of HR and biofilm formation of Xoo.

Key words:Keywords: Xanthomonas oryzae pv. oryzae; c-di-GMP; vieAxoo; gene deletion; functional characterization