大豆过氧化物酶－过氧化氢－碘化钾体系杀菌作用

Bactericidal effect of soybean peroxidase - hydrogen peroxide - potassium iodide system

摘要:

摘要： [目的] 研究豆壳过氧化物酶（SBP）-H2O2-KI三元体系杀菌作用及其可能的杀菌机制。[方法] 以细菌生长的浊度（OD600）为指标检测SBP-H2O2-KI体系的抑菌作用；以活细胞计数（CFU）为指标检测SBP-H2O2-KI体系的杀菌作用；以最低抑菌浓度（MIC）为指标检测亚致死剂量的SBP-H2O2-KI体系作用下连续传代细菌的敏感性变化；以物理和化学方法检测SBP-H2O2-KI体系中活性氧基团等功能基团的生成与否，以期解释SBP-H2O2-KI体系的杀菌机制。 [结果] SBP

关键词:关键词： 过氧化物酶，细菌，抗性，过氧化物酶－H2O2－KI，机制

Abstract:

Abstract: [Objective] To study the bactericidal effect and the possible mechanisms of the three components system [soybean peroxidases (SBP)-hydrogen peroxide (H2O2)-potassium iodide (KI), SBP-H2O2-KI]. [Methods] The inhibition and bactericidal effect of SBP-H2O2-KI system to bacteria were detected by OD600 and the number of live bacteria (CFU). The sensitivity was tested by comparing the minimum inhibitory concentration (MIC) of bacterial cultures before and after cultured under sub-lethal dose of SBP-H2O2-KI system. Oxidizing activity groups were detected with physical and chemical methods in order to explain the bactericidal mechanisms of SBP-H2O2-KI system. [Results] SBP-H2O2-KI ternary system had rapid and high efficient bactericidal effect to a variety of bacterial strains in just several minus. The MICs had no significant changes when bacterial cultures continuously cultured in sub-lethal dose of SBP-H2O2-KI system, and no resistance / tolerance mutant strains could be isolated from them. Both physical and chemical test results showed that no hydroxyl radical produced in SBP- H2O2-KI reaction system, chemical test results showed that no superoxide anion but a singlet oxygen and iodine produced in SBP-H2O2-KI reaction system. [Conclusion] These results suggested that singlet oxygen and iodine or the iodine intermediate state may possible be the main sterilization factors for SBP-H2O2-KI system, and hydroxyl radical and superoxide anion not. In addition, the both characteristics of SBP-H2O2-KI system: rapid and high efficient bactericidal effect, and bacteria difficultly resisting to it, indicated it would have a good potential application in medical and plant protection area.

Key words:Keywords: Peroxidase, Bactericidal mechanism, Resistance, Peroxidase-H2O2-KI, Mechanism