一株黑曲霉(Aspergillus niger)对羟基磷灰石合成的诱导

Induced synthesis of hydroxyapatite by Aspergillus niger

摘要:

摘要：【目的】研究利用真菌诱导的方式合成羟基磷灰石（HAP）。【方法】采用含不同浓度Na2HPO4和CaCO3的PDA（Potato Dextrose Agar Medium）液体培养基，研究黑曲霉作用诱导HAP合成，并用透射电镜（TEM）观察诱导形成的矿物晶体形态和结构、用X射线衍射（XRD）法确定矿物种类。【结果】主要研究结果如下：（1）在含有合适浓度的Na2HPO4和CaCO3的PDA液体培养基中，接入黑曲霉可以诱导HAP晶体的合成。（2）黑曲霉对HAP合成的诱导作用跟反应时间有关，反应时间越长，越

关键词:关键词：黑曲霉，羟基磷灰石，诱导作用，生物成矿

Abstract:

Abstact:[Objective] The research object is to induce hydroxyapatite (HAP) synthesis using fungus. [Methods] Using the PDA (Potato Dextrose Agar Medium) liquid medium containing different concentrations of Na2HPO4 and CaCO3 to study the way Aspergillus niger synthesize HAP, and observe the induced mineral crystal structure and analysis the induced mineral type with transmission electron microscopy (TEM) and X-ray diffraction (XRD). [Resluts] The main results are as follows: (1) A. niger can induce HAP synthesis in PDA liquid medium with the proper concentration of Na2HPO4 and CaCO3. (2) The reaction of A. niger induced HAP synthesis depends on the time of the response system. Longer time is more advantageous in producing HAP. [Conclusion]The main reason for A. niger inducing HAP crystals formation are as follows: fungal metabolism produces the acidic substances to dissolve CaCO3 and the growth mycelia absorbing Ca2＋ lead to Ca2＋enriched on the surface, to promote the production of secondary mineral apatite and further transform into HAP in the mycelia spheres. Because the response condition for bio-induction HAP is temperate, the preparation craft is simple, the cost is low, the method has the potential application prospect.

Key words:Keywords: Aspergillus niger, hydroxyapatite, Induction, Biological mineralization