广州地区嗜肺军团菌环境分离株的基因序列分型分析

Sequence-based typing of enviromental Legionella pneumophila isolates in Guangzhou

摘要:

摘要:【目的】研究广州市嗜肺军团菌的基因特征,对来自不同水域环境的嗜肺军团菌进行分子分型研究。【方法】选择嗜肺军团菌的7个基因flaA、asd、mip、pilE、mompS、proA和neuA 作为目的基因, 对在2006-2009年间广州地区分离的44株嗜肺军团菌进行PCR扩增和测序，并将核苷酸序列上传至欧洲军团菌病感染工作组（EWGLI）数据库进行比对,得到基因型别（Sequence type, ST），对结果进行基因序列分型（Sequence-Based Typing, SBT）和系统进化分析。【结

关键词:关键词: 嗜肺军团菌，基于基因序列分型，分子分型

Abstract:

Abstract: [Objective] To characterize the genes of Legionella pneumophila isolated from different water source in Guangzhou from 2006 to 2009. To genotype the strains by using sequence-based typing (SBT) scheme. [Methods] In total 44 L. pneumophila strains were identified by SBT with 7 diversifying genes of flaA、asd、mip、pilE、mompS、proA and neuA. Analysis of the amplicons sequence was taken in the European Working Group for Legionella Infections (EWGLI) international SBT database to obtain the allelic profiles and sequence types (STs). Serogroups were typed by latex agglutination test. [Results] Data from SBT revealed a high diversity among the strains and ST01 accounts for 30% (13/44). Fifteen new STs were discovered from 20 STs and 2 of them were newly assigned (ST887 and ST888) by EWGLI. SBT Phylogenetic tree was generated by SplitsTree and BURST programs. [Conclusion] High diversity and specificity were observed of the L. pneumophila strains in Guangzhou. SBT is useful for L. pneumophila genomic study and epidemiological surveillance.

Key words:Keywords: Legionella pneumophila, Sequence-Based Typing(SBT), genotype