产D-阿拉伯醇菌株的筛选、鉴定及其产D-阿拉伯醇条件的优化

Isolation and identification of a novel Candida sp. H2 producing D-arabitol and optimization of D-arabitol production

摘要:

摘要：【目的】产D-阿拉伯醇的耐高渗酵母的筛选、鉴定和产D-阿拉伯醇条件的优化。【方法】通过电镜、Biolog GN、(G+C)含量和26S rDNA D1/D2区序列分析法对所获得的菌株进行了描述。通过红外光谱、核磁共振氢谱和碳谱、质谱以及旋光度实验鉴定纯化产物的结构。通过单因素实验优化产D-阿拉伯醇的发酵条件。【结果】本文筛选得到一株产D-阿拉伯醇的新型菌株，经鉴定属于假丝酵母属并命名为Candida sp. H2。200 mL摇瓶发酵生产D-阿拉伯醇的单因素优化实验表明，最适发酵条件为：葡萄糖250

Abstract:

Abstract: [Objective] To isolate a new osmophilic yeast for producing D-arabitol and research its optimal fermentation conditions for highest yield of D-arabitol from glucose. [Methods] The isolated strain was characterized by electron microscopy, Biolog (GN) test, G+C content measurement and 26S rDNA D1/D2 domain sequences analysis. The purified fermentation product was identified by IR, 1H-NMR, 13C-NMR, MS and optical rotation analysis. Then the fermentation conditions for D-arabitol production were optimized. [Results] A new osmophilic yeast was isolated and identified as Candida sp. H2. Through the single factor experiment, the optimum conditions of 250 g/L glucose, 10 g/L yeast extract, initial pH 6.0, 35 °C of culture temperature, 200 rpm of agitation, 200 mL medium in a 1000-mL flask of broth content, 1% (v/v) of inoculum size, 96 h of fermentation time were achieved. Based on the conditions above, weight yield of 35% (86.55 g D-arabitol from 250 g glucose) was obtained and 10% higher than the conditions not optimized. [Conclusions] Candida sp. H2 was a novel strain for producing D-arabitol and valuable for further study.