一株石油烃降解菌新种Marinobacter sp. PY97S的鉴定
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国家自然科学基金(40906062;41076108);公益性行业科研专项经费项目(200705011);国家海洋局青年海洋科学基金(2009114);海洋一所基本科研业务费专项资金(2010G23)


Identification and characterization of a novel hydrocarbon-degrading Marinobacter sp. PY97S
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Supported by the National Natural Science Foundation of China (40906062;41076108), by the Public Welfare Project of Marine Science Research (200705011), by the Marine Science Foundation for the Youth (2009114) and by the Project of Fundamental Science Research, First Institute of Oceanography (2010G23)

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    摘要:【目的】为了对1株从黄海沉积物中分离到的石油烃降解菌新种PY97S进行分类学鉴定。【方法】采用16S rRNA基因序列同源性分析、生理生化指标测定、抗生素抗性实验、DNA G+C含量测定、全细胞脂肪酸组成测定、碳源利用实验、呼吸醌测定以及DNA杂交实验等多种方法对该菌株进行鉴定,并通过降解实验测定其对烷烃的利用情况。【结果】菌株PY97S为海杆菌(Marinobacter),革兰氏阴性,接触酶阳性,氧化酶阳性,主要呼吸醌为Q-9。在GenBank中与其16S rRNA基因序列相似度最高的模式株为Marinobacter koreensis DD-M3T(96.93%),两者DNA-DNA同源性仅为46.7%。菌株PY97S的温度生长范围为15℃-35℃(最适为30℃),NaCl浓度生长范围是0%-10%(最适为0%),初始pH生长范围为pH 6.0-9.0(最适为初始pH7.0)。该菌株可以利用多种糖类和有机酸类的碳源,并对氨苄青霉素、氧哌嗪青霉素等多种抗生素敏感。其DNA G+C含量为48.2 mol%。其主要脂肪酸组成为2-methyl C15:0(29.97%)、C16:1ω7c(27.22%)、C12:0(22.22%)和C16:1ω9c(5.73%)。【结论】菌株PY97S是1株能够降解多种多环芳烃和烷烃的海洋石油烃降解菌新种,具有应用到溢油污染海洋环境生物修复的潜力。

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    Abstracts: [Objective] To identify and characterize a hydrocarbon-degrading bacterium isolated from the sediment of the Yellow Sea. [Methods] We used 16S rRNA gene sequences based phylogenetic analysis, physiological and biochemical characterization, DNA G+C content assaying, determination of cellular fatty acids, testing of carbon sources and respiratory lipoquinone and experiment of DNA–DNA relatedness. Its capability of degrading aliphatic hydrocarbons in ONR7a media supplemented with nine n-alkanes, separately, as sole source of carbon and energy was further determined. [Results] The Gram-negative isolate PY97S was a member of the genus Marinobacter, catalase- and oxidase-positive, and with Q-9 as its predominant respiratory lipoquinone. The similarity between its 16S rRNA gene and that of its most closely related type strain in GenBank Marinobacter koreensis DD-M3T was 96.93%, and their level of DNA relatedness was 46.7%. The appropriate temperature for its growth ranged from 15℃ to 35℃ with the optimum of 30℃, the appropriate initial acidity from pH 6.0 to 9.5 with the optimum of pH 7.0, and the appropriate salinity (NaCl) from 0% to 10% with the optimum of 0%. It metabolized many carbohydrates and organic acids and was sensitive to diverse antibiotics including ampicillin and piperacillin. The G+C content of its genomic DNA was 48.2 mol%. The major fatty acids were 2-methyl C15:0 (29.97%), C16:1ω7c (27.22%), C12:0 (22.22%) and C16:1ω9c (5.73%). [Conclusion] The isolate PY97S was identified as a petroleum hydrocarbon-degrading novel species of genus Marinobacter, holding the potential of being applied in the bioremediation of oil spill.

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李倩,崔志松,赵爱芬,高伟,郑立. 一株石油烃降解菌新种Marinobacter sp. PY97S的鉴定. 微生物学报, 2011, 51(5): 648-655

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  • 收稿日期:2010-11-09
  • 最后修改日期:2010-01-31
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