乳杆菌吸附苯并芘的特性

Binding of benzo(a)pyrene by Lactobacilli strains

摘要:

摘要:【目的】探讨植物乳杆菌(Lactobacillus plantarum)121 和戊糖乳杆菌(Lactobacillus pentosus)ML32 的苯并芘吸附作用与机制。【方法】采用高效液相色谱检测菌体对苯并芘的吸附率。【结果】菌株121 和ML32对苯并芘的吸附率分别为65.9%和64.9%，这种吸附特性与菌体活力无关，随培养时间延长、温度提高以及细胞浓度的上升而增加。菌株121 和ML32 的吸附率在pH4和5 时达到最大，分别为87.6% 和89.0%。当培养液中Ca2+或Mg2+浓度大于

Abstract:

Abstract:［Objective］We studied the ability of Lactobacillus plantarum 121 and Lactobacillus pentosus ML32 to bind benzo(a) pyrene．［Methods］ The percentage of benzo (a) pyrene bound by the lactobacilli strains was quantitated by HPLC after bacterial cells and benzo (a) pyrene were co-incubated in MRS media at 37℃ for 4 h．［Results］The percentage of benzo (a) pyrene-binding was 65.9% for 121 and 64. 9% for ML32．Physical factors affecting binding ability included incubation time，temperature，bacterial cell viability，pH and concentrations of Ca2 + and Mg2 + ． Different chemical and enzymatic treatments to cells affected the binding of the two strains to benzo(a) pyrene． The simulation of gastrointestinal environments showed that the binding ability of the two strains depended largely upon pH and bile salt concentrations，but less upon treating time． Trypsin had only influence on the ability of 121 to bind benzo(a) pyrene．The presence of benzene in washing cell impaired the ability of the two strains to bind benzo (a) pyrene．［Conclusions］Strains 121 and ML32 had potential to bind benzo(a) pyrene．