禽致病性大肠杆菌IMT5155 疑似毒力基因在人源及动物源大肠杆菌中的分布

Distribution of putative virulence genes of Avian pathogenic Escherichia coli IMT5155 in E．coli strains isolated from human and animal

摘要:

摘要:【目的】检测禽致病性大肠杆菌IMT5155 自分泌黏附素基因等具有代表性的疑似毒力基因在不同来源大肠杆菌中的分布，为进一步研究其致病机理提供依据。【方法】采用PCR 和Dot blot，检测疑似毒力基因在不同地区(101 株大肠杆菌中国分离株和121 株大肠杆菌德国分离株)、不同来源(人源、禽源及猪源)大肠杆菌中的分布，并分析其和大肠杆菌系统进化分群的关系。【结果】自分泌黏附素基因B11 等11个疑似毒力基因在禽致病性大肠杆菌中分布率较高，阳性率分别为:A1 36. 4% (32/88 )、A8 5

关键词:关键词: 禽致病性大肠杆菌，疑似毒力基因，分布

Abstract:

Abstract:［Objective］We investigated the distribution of autotransporter adhesin gene B11 and other putative virulence genes of APEC IMT5155，which contributed to study the pathogenic mechanism of Avian pathogenic Escherichia coli (APEC)．［Methods］PCR and Dot blot were used to detect the distribution of putative virulence genes in E．coli strains isolated from different country (101 Chinese strains and 121 German strains)，which were from different sources (human，avian and pig)．The association of E． coli phylogenetic group and distribution of putative virulence genes was also analyzed．［Results］The putative virulence genes were most prevalent among APEC isolates，36. 4% (32/88) of APEC isolates harboring A1，53.4% (47/88) for A8，63.6% (56/88) for A10，37.5% (33/88) for B11，59.1% (52/88) for F3．The isolates positive for putative virulence genes were linked to E． coli phylogenetic group B2． Among Neonatal meningitis E．coli isolates，60%,80% and 80% were positive for putative virulence genes D1，E9 and F11，respectively． However，all the Neonatal meningitis E． coli strains were negative for B11．Conclusion］ Autotransporter adhesin gene B11 and other putative virulence genes were associated to APEC． However，putative virulence genes D1，E9 and F11 were associated to Neonatal meningitis E． coli，which indicated that APEC might be a virulence gene reservoir for Neonatal meningitis E.oli．

Key words:Keywords: Avian pathogenic Escherichia coli，putative virulent genes，distribution