摘要:VBNC( viable but non-culturable) 是指处于“活的但非可培养”状态的微生物,此微生物体的细胞仍有代谢活性,但用常规方法无法分离培养。本文阐述了VBNC 状态菌的形成机理、转变与种类、复苏、研究意义及其应用展望。并报道了我们在十余年间针对生态环境中处于VBNC 状态菌的复苏、可培养化、系统进化关系及潜在功能等方面的一些研究成果,拟为微生物资源的开发与应用提供新的科学依据。
Abstract:
Abstract:The viable but non-culturable ( VBNC) is a microbial state,in which microbial cells are metabolically active but cannot be cultivated by routine methods. In this article,we address the formation mechanism,change and variety,resuscitation,research significance and application prospects of VBNC state in bacteria. Furthermore,we report our research findings on VBNC state of bacteria in the past 10 years,including resuscitation,culturable,phylogenetic relationship and potential functions.