南海北部陆坡神狐海域HS-PC500 岩心微生物多样性

Microbial diversity in sediments of core HS-PC 500 from Shenhu Area，northern South China Sea

摘要:

摘要:【目的】本文研究南海北部陆坡神狐HS-PC500 重力活塞岩心沉积物中微生物多样性。【方法】使用吖啶橙染色法计数沉积物中微生物丰度;提取沉积物微生物总DNA，使用特异性引物扩增古菌及细菌16SrRNA 基因序列;对克隆文库进行系统发育分析。【结果】系统发育分析显示表层PC500-1 (0 － 5 cm belowsea floor，bsf) 古菌以C3 为主要类群，占该层总序列的25. 6% ; 中层PC500-6 ( 350 － 355 cm bsf) 和底层PC500-11 (790 － 795

Abstract:

Abstract:［Objective］We studied the microbial diversity in the sediments of different depth in a gravity piston core HSPC500 from Shenhu Area，the northern of South China Sea． ［Methods］ Total DNA was extracted from the sedimental materials; the archaeal and bacterial 16S rRNA gene sequences were amplified． The clone libraries were used to analyze the microbial systematic development． ［Results］Group C3 was the predominant archaeal group in the top layer(0 － 5 cm bsf) sediments，and the Marine Benthic Group(MBG) -B group became predominant with depth，reaching 38. 9% and 62. 5% in the middle ( 350 － 355 cm bsf ) and bottom ( 790 － 795 cm bsf ) sediments． Some belonged to MBG-A，Miscellaneous Crenarchaeotic Group(MCG) ，Thermoprotei，Novel Group Crenarchaeota( NGC) ，Halobacteriales，MBGE，South African Gold Mine Euryarchaeotic Group( SAGMEG) ． Proteobacteria was the dominant bacterial group in the top of the core，but became minor deeper within the sediments． As depth increased，Chloroflexi and candidate division JS1 became the predominant groups and reached up to 28. 1% ，29. 2% and 39% ，24. 7% ． Other sequences respectively belonged to Nitrospirae，Actinobacteria，Acidobacteria，candidate division OP8，Spirochaetes，candidate division TM6，Deferribacteres and Plantomycete． ［Conclusion］ Down-core variation in microbial abundance in sediments of HS-PC500 was consistent with the changes of methane concentration in the same core; the lower microbial abundance might be probably due to lower total organic carbon ( TOC) in sediments; However，microbial diversity was relatively high and community structure varied apparently with depth; the community was dominated by clusters that was dominated in sulfate reduction condition，suggesting that microbial etabolization played very important role in the material cycle of marine sediments．