一株抗水稻纹枯病菌的解淀粉芽胞杆菌分离与鉴定

Isolation and identification of a Bacillus amyloliquefaciens YB-3 against Rhizoctonia solani

摘要:

摘要: 【目的】筛选对水稻纹枯病菌(Rhizoctonia solani) 具有强拮抗作用的细菌菌株。【方法】用指示菌法筛选拮抗菌株; 通过形态观察、生理生化实验、Biolog 及16S rDNA 序列分析鉴定目标菌株; 利用平板双向培养法和滤纸片扩散法测定抑菌谱及拮抗性质。【结果】分离到一株高活力的水稻纹枯病菌拮抗菌株YB-3，该菌株属于解淀粉芽胞杆菌(Bacillus amyloliquefaciens) ; 菌株YB-3 对常见的14 株病原真菌和7 株细菌具有较强的拮抗作用，并发现其对亲缘关系较近

Abstract:

Abstract: ［Objective］An antagonistic bacterial strain YB-3 against Rhizoctonia solani was isolated from soils．［Methods］ Antagonistic strains were isolated by a reporter strain method． YB-3 was identified based on morphology observation，physiological and biochemical characterizations，Biolog，G + C content and 16S rDNA sequence analysis．The antagonistic spectrum and the properties of the inhibitor produced by Bacillus amyloliquefaciens YB-3 against plant pathogenic fungi and bacteria were investigated by means of plate two-way cultivation and disc diffusion method．［Results］ The strain YB-3 against Rhizoctonia solani was identified as Bacillus amyloliquefaciens． The antagonistic results showed that it had distinctively inhibitive effects on 14 pathogenic fungi and 7 bacteria． In addition，it also had inhibitive effects on strains from genus Bacillus to which YB-3 belongs． Antagonistic properties of B．amyloliquefaciens YB-3 was thermostable，acid resistant，and protease sensitive． ［Conclusion］ Bacillus amyloliquefaciens YB-3 was isolated and characterized which had distinctively inhibitive effects on Rhizoctonia solani and had broad-spectrum，highly efficient to plant pathogens．