艾丁湖沉积物放线菌多样性

Studies on the Actinobacterial Diversity in Aiding Lake Sediments

摘要:

摘要: 【目的】为了研究新疆艾丁湖低海拔、高盐环境中的放线菌多样性。【方法】本研究应用基于16S rRNA基因序列系统发育分析的免培养方法和选择性分离培养方法相结合的方式对艾丁湖沉积物样品进行放线菌多样性分析。利用放线菌特异性引物扩增16S rRNA 基因并构建了16S rRNA 基因克隆文库，对文库中的插入序列进行RFLP( Restriction Fragment Length Polymorphism 限制性片段长度多态性) 分析。采用不同盐浓度的9 种选择性培养基分离样品中的放线菌。【结果】通过对

关键词:关键词: 艾丁湖，放线菌多样性，免培养方法，选择性分离

Abstract:

Abstract: ［Objective］ This study aims at investigating the diversity of actinobacteria in Aiding Lake，a hypersaline lake and the lowest land point in China． ［Methods］ The diversity of actinobacteria in the sediment from Aiding Lake was investigated by culture － independent method based on phylogenetic analysis of 16S rRNA gene sequences and selective isolation． Specific primers were used to amplify the actinobacterial 16S rRNA gene，and corresponding clone libraries were constructed for the sediment samples． Different clones selected on the basis of HaeⅢ digestion patterns were sequenced．Nine selective media with different salinities were used to isolate actinobacteria from the sediment samples．[Results]The analysis of 16S rRNA gene sequences showed that 273 clone sequences belonged to subclasses Actinobacteridae(208) ，Acidimicrobidae (13) and Rubrobacteridae (52).The dominant actinobacteria was genus Rothia，which accounted for 37% of total clones． The similarity between 45. 8% of 273 detected sequences and published sequences were less than 97% ， which might represent new taxa． Some sequences，which formed several distinct clades in phylogenetic tree may represent new taxonomical groups of actinobacteria． Fifty-five strains were isolated by different selective media． They belonged to six suborders of the order Actinomycetales，of which Streptomyces and Nocardiopsis were the dominant groups．Six potential new species were obtained．[Conclusion] Aiding Lake harbors abundantactinobacteria，including large number of unknown actinobacterial groups．

Key words:Keywords: aiding lake，actinobacterial diversity，cultural-independent method，selective isolation