表达蝎毒素的重组斜纹夜蛾核型多角体病毒(SpltMNPV)的构建及毒力

Construction and toxicity the recombinant SpltMNPV expressing the scorpion toxin gene

摘要:

摘要: 【目的】开发高毒力的重组斜纹夜蛾核型多角体病毒( Spodoptera litura multicapsid nucleopolyhedroviruse，SpltMNPV) 杀虫剂。【方法】构建编码蜕皮激素UDP-葡萄糖基转移酶(ecdysterioid UDP-glucosyl transferase gene，egt) 基因缺失并插入东亚钳蝎神经毒素(B． martensi Karsch，BmK ITa1) 基因的重组转移载体，重组转移载体与SpltMNPV Ⅱ基因组DNA 共转染斜纹夜蛾细胞

关键词:关键词: 斜纹夜蛾核型多角体病毒(Spodoptera litura multicapsid nucleopolyhedroviruse，SpltMNPV)，东亚钳蝎神经毒素基因(B．martensi Karsch，BmK ITa1) ，蜕皮激素UDP-葡萄糖基转移酶基因(ecdysterioid UDPglucosyl transferase gene，egt)，重组杆状病毒

Abstract:

Abstract: ［Objective］ To develop a high toxic recombinant Spodoptera litura multicapsid nucleopolyhedroviruse (SpltMNPV) insecticide． ［Methods］We constructed a recombinant transfer vector that was characterized by disrupting of ecdysterioid UDP-glucosyltransferase (egt) gene and expressing the mature peptide of the Chinese scorpion，B．martensi Karsch(BmK ITa1) gene at the control of ie-1 promoter． The transfer vector and the SpltMNPV II DNA cotransfected the SpLi cells． Recombinant viruses were purified by the end point dilution and fluorescent spot purification．［Results］We successfully screened the recombinant SpltMNPV-Δegt-Pph-egfp-ie-1-BmK ITa1 of which the egt gene was knocked out and expressed the mature peptide of the BmK ITa1 gene at the control of ie-1 promoter． Bioassays showed that，compared to the wide-type SpltMNPV，the speed of the recombinant virus killing the S． litura (LT50) increased by 0.7－0.8 days．［Conclusion］ The insecticidal effect of SpltNPV could be increased by inserting the foreign gene，which provided a further opportunity to develop the SpltNPV into commercially viable products to control the S． litura．

Key words:Keywords: Spodoptera litura multicapsid nucleopolyhedroviruse (SpltMNPV)，B.martensi Karsch (BmK ITa1)，ecdysterioid UDP-glucosyltransferase (egt)，recombinant baculovirus