黑龙江大豆胞囊线虫胞囊可培养细菌多样性

Diversity of bacteria isolated from cysts Heterodera glycines in Heilongjiang

摘要:

摘要:【目的】了解黑龙江省大豆田大豆胞囊线虫胞囊可培养细菌的多样性。【方法】运用稀释平板法和16SrDNA 基因序列的系统发育分析对胞囊可培养细菌多样性进行研究。【结果】用NA 培养基从胞囊上分离90株具有不同菌落形态的细菌。16S rDNA序列分析结果表明:90 株菌株分属于7个属22个种。46株属于变形菌门γ亚群(Gammaproteobacteria)，32 株属于厚壁菌门(Firmicutes)，10 株属于变形菌门β亚群(Betaproteobacteria)，2 株属于变形菌门ɑ亚群(Alphaproteobacteria)。假单胞菌属(Pseudomonas)和芽孢杆菌属(Bacillus)为优势菌属。【结果】黑龙江省大豆胞囊线虫胞囊中存在丰富的细菌物种多样性，这些细菌对大豆胞囊线虫可能具有一定的生理生态作用。

Abstract:

Abstract:［Objective］To understand the bacterial diversity isolated from the cysts of Heterodera glycines in the soybean field in Heilongjiang Province．［Methods］Bacteria were isolated from cysts on nutrient agar plates using dilution plate method and further identified by phylogenetic analysis based on 16S rDNA gene sequences． ［Results］Totally 90 bacteria strains with different colony morphology were selected on nutrient agar plate and their phylogenetic features were analyzed based on the partial 16S rDNA sequences． In total 7 genera and 22 species were identified，including 46 strains in Gammaproteobacteria (51.1%)，32 in Firmicutes (35.6%)，10 in Betaproteobacteria (11.1%)，and 2 in Alphaproteobacteria (2.2%)． The dominant bacteria species were Pseudomonas and Bacillus．［Conclusion］There was abundant species diversity of bacteria isolated from cysts Heterodera glycines in Heilongjiang，and these bacteria may play a physical and ecological roles in nematodes．