五种动物粪便纯培养放线菌的多样性及生物活性

Diversity and bioactivity of culturable actinobacteria from animal feces

摘要:

摘要:【目的】以资源利用为出发点，研究了五种动物粪便放线菌的多样性及生物活性。【方法】从云南野生动物园采集5 种半野生动物的新鲜粪便样品，用5 种培养基分离其中的放线菌，将放线菌鉴定到属，测定纯培养放线菌的抗菌活性，抗肿瘤活性等。【结果】结果表明，所试5 种动物粪便放线菌的种类非常丰富，组成各不相同，以链霉菌和微球菌占优势，可能存在大量未知放线菌;粪便放线菌的抗菌活性，抗肿瘤活性，酶活性，分解纤维素、角蛋白的活性非常普遍，尤其是具有抗肿瘤活性的菌株比例较大;产生的具有生物活性的次生代谢产物也是多种多样的。【结论】因此，粪便放线菌与土壤，海洋及植物的放线菌一样，是开发药物、农药和其他产品的重要来源之一，应当加强粪便放线菌的研究和保护利用。

Abstract:

Abstract:［Objectives］In order to provide new source for discovering new lead compounds of drugs and other products，the diversity and some bioactivities of culturable actinobacteria in animal feces were studied． ［Methods］Five animals' fecal samples were collected from Yunnan Wild Animal Park． The pure cultures of actinobacteria were isolated from these samples by using 5 different media． The 16S rRNA gene sequences of 119 selected strains were determined; the phylogenetic analysis was carried out; and antimicrobial and anti-tumor activities were determined by using agar diffusion method，tumor cell lines k562and HL60 respectively． ［Results］In total 20 genera of actinobacteria from the 5 animals' feces were identified． Many strains inhibited Bacillus subtilis，Staphylococcus lentus，Mycobacterium tuberculosis，Candida albicans and Aspergillus niger． Some strains presented antitumor activities． Some known secondary metabolites and Sannastatin，a novel macrolactam polyketide glycoside with bioactivities，were isolated and identified． ［Conclusion］Fecal actinobacteria are a new potential source for discovering drug lead and other industry products．