口腔乳酸杆菌的分离及其益生特性

Characterization of a probiotic Lactobacillus strain isolated from oral cavity

摘要:

摘要:【目的】从口腔环境中筛选具有潜在益生特性的乳酸杆菌，用于防治口腔疾病的益生菌疗法。【方法】利用选择性培养基从健康志愿者的唾液和牙菌斑样品中筛选得到乳酸杆菌，然后验证他们对龋齿致病菌变异链球菌生长的抑制作用。同时考察分离得到的微生物是否具有可以定植或在口腔环境中生存的特性。【结果】本研究从牙菌斑样品中分离得到一株发酵乳杆菌Y29。该菌能够抑制变异链球菌的生长，并有自聚集和与其他口腔微生物共聚集形成生物膜的能力。此外，发酵乳杆菌Y29可耐受1.0 mg/mL 溶菌酶和140μg/g过氧化氢，有利于其在可能含有多种抑菌物质的口腔动态环境中生存。【结论】发酵乳杆菌Y29 在防治龋齿和保证口腔健康方面具有潜在的益生特性。

关键词:关键词:发酵乳杆菌，抑菌活性，胞外多糖，益生菌，口腔健康

Abstract:

Abstract:［Objective］ From oral cavity we isolated and characterized probiotic lactobacilli that could probability be applied to therapy and prevention of oral diseases. ［Methods］Lactobacillus strains were isolated by plating the saliva and dental plaque of healthy donors on selective medium．Then the target strains were tested for inhibiting the growth of a Streptococcus mutans strain belonging to cariogenic pathogen species．Other properties such as production of extracellular polysaccharide and resistance to the antibacterial substances were also investigated．［Results］ Lactobacillus fermentum Y29，a strain with antimicrobial activity against Streptococcus mutans，was obtained from dental plaque．This strain was an extracellular polysaccharide producer，which corresponds to its aggregation ability． Moreover，L． fermentum Y29 showed resistance to 1.0 mg/mL lysozyme and 140 μg/g hydrogen peroxide that may guarantee its persistence in the complex oral niche． ［Conclusion］ Probiotic properties were characterized of an oral isolate L．fermentum Y29，which provided a possibility for its application in prevention and treatment of oral diseases．

Key words:Keywords: Lactobacillius fermentum，antimicrobial activity，extracellular polysaccharide，probiotics，oral health